Cross-interactions between Norepinephrine, Methicillin-Resistant Staphylococcus aureus and Human Osteoblast Cells in Culture Conditions

Objective: The role of norepinephrine (NE) on growth, adhesion and invasion methicillin-resistant Staphylococcus aureus (MRSA) ATTC 43300 was examined in human osteoblast (HOB) cells. effects NE and/or MRSA the viability cell death pathways HOB cells were also investigated. Furthermore, alterations bacterial response to oxidative stress (H2O2) analyzed presence/ absence NE. Materials Methods: Bacterial growth detected spectrophotometrically. colony counting method for adhesion-invasion. alteration determined by methyl thiazolyl diphenyl-tetrazolium bromide (MTT) assay. microscopically using acridine orange-ethidium dual staining dichlorofluorescein-diacetate (DCF-DA) dye. H2O2 investigated agar dilution. Results: bacterium not affected presence decreased (p<0.0001) while high-level induced (p=0.013). reduced (p<0.001). co-existence caused necrosis more than apoptosis (p<0.05). did alter stress. Conclusion: Norepinephrine has different biological properties both